FastQCFastQC Report
星期二 11 七月 2023
Z3.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZ3.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9942842
Total Bases497.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA241791124.31810743849696Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC4944954.973376827269306Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG3541423.56177841305333No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1736091.7460701879804585Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1419461.4276199903407898Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGATCGGAAG1392681.400686041274718No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA1360271.3680897272630903No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC1220961.227978881691975No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG1220001.2270133629801216No Hit
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1074211.0803852661039972Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC931480.9368347601219048Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA865170.8701435665979607Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC699550.7035714738301182Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC648610.6523386371824073Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT599230.6026747684414577No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC586020.5893888286668942Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC568120.5713859276854646No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG567170.5704304664601932Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC557730.5609361991269699Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC556890.5600913702540984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA543850.5469764077514255No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT508380.5113025028457658No Hit
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCA447650.4502233868344685Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA415540.41792879742029493Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC398080.40036842584846466Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCGTTACTATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA359270.36133532042448224Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC342180.34414707585617876Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG340890.342849660087126No Hit
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC339780.3417332790765457Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC330700.33260108126026744No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC325130.32699906123420247No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC313180.3149803647689463Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC312410.31420593830214744No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA306870.3086340907358278Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC303060.3048021883481604Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA282870.28449612293949755Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCA278080.27967858686681335Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAC271470.2730305882362407No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT265770.26729782088461224No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA257260.25873889980349685No Hit
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG237470.23883513385810615Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC224710.22600178097972393No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC224070.22535810183848845Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG221770.22304487992467348No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG220190.22145579704474838No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC216300.2175434347644265No Hit
GTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCG216120.21736240000595403No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG213360.21458653370937608No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATG207630.2088235938980022TruSeq Adapter, Index 1 (97% over 37bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCCCACGTCTGAACTCCA203510.20467990942629882Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC200870.20202473296870252Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACA184670.18573160470617958No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC181900.1829456809230198No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC175960.17697153389342807Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG175830.1768407865678646Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAG171410.17239537749870712No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC169630.17060514488714595No Hit
TGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCA168630.16959939622896553No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAAGATCGGAAGA168240.16920715425227517No Hit
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACACGTCTGAACT166320.16727611682856872Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACATTAGTGTCAC165090.16603904597900682No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC163430.1643695032064273Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACAAGATCGGAAG162500.1634341569543195No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTC161000.16192553396704887No Hit
CAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCT159910.1608292679296322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGTATGCCG157950.15885800055959856TruSeq Adapter, Index 1 (96% over 33bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA154570.1554585700949487Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC153130.1540102920271689No Hit
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC152160.15303471582873387Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGGATGCCG151640.15251172652648004TruSeq Adapter, Index 1 (96% over 33bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA147530.1483780995413585Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA146200.14704045382597852Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGC144080.14490826667063603No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTC142620.1434398736296926No Hit
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA142590.14340970116994717Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA138170.1389642921007897Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGGATGGCG136010.13679187499911996TruSeq Adapter, Index 1 (96% over 33bp)
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC132140.1328996276919617Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA130810.13156198197658173No Hit
GAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCAC129960.1307070956171284No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC126500.1272272052598241Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACAC122650.12335507292582945No Hit
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA121320.12201742721044949Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGACTGACCTGAAATGAAGAGAATACTCATTGCTGATCAGATCGGAAGA120830.12152461036794109No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGTAGATCGGAAGAGCAC117180.11785362776558252No Hit
GTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCC115040.1157013256370764No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC114770.11542977349936769No Hit
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA112440.1130863791258073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCACTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA110520.11115534170210085Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACCTCTGAACTCCA108600.10922430427839444Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG106380.1069915422572339No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCGCGTATGCCG103980.10457774547760086TruSeq Adapter, Index 1 (96% over 33bp)
AAACCGTTACCATTACTGAGTTTAGTAGATCGGAAGAGCACACGTCTGAA103460.10405475617534705Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGA101920.10250590324174919Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGATCGGAA101230.1018119366676047No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGTA100730.10130906233851449TruSeq Adapter, Index 1 (97% over 37bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA100100.1006754406838608Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTTGGACTACATATGGTTGAGGGTTGTAGATCGGAAGAGCACACGTCT100010.10058492330462458Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)