FastQCFastQC Report
星期二 11 七月 2023
Z2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameZ2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10535324
Total Bases526.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA266651425.310223017346217Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC8379847.954041090715387Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG3852023.6562900201265762No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC2510632.3830591256614415Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1869481.7744874291478838Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1712111.625113760146342Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1683311.5977771542669212Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC1516751.4396804502642728No Hit
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1495891.4198803947557759Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1353261.284497752513354Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1239081.1761195004539016Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1178221.1183519367795427Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC945370.8973335798690196Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCA831640.789382462276433Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA759250.7206707643732647Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC735990.698592658374816Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCA710360.67426497751754Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC702200.6665196058517042Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC573120.5439984570004681Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG536650.5093815814302436No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT524810.4981431990131485No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC518260.4919260195509887Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC459430.4360853069160474Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC413570.3925555588038868Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG398280.3780424788074861Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACTATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA379660.36036860375627744Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC376560.35742612187342315Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC356970.33883153474919236No Hit
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC340670.32335977517160364Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA329290.31255801909841596No Hit
TACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGTCTGAACTCC318250.30207898684463813Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC302000.28665468665225674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCC300390.2851264944485808Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA295680.28065582036205056Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA290140.27539732048107873Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG283670.26925607603525054No Hit
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA279170.26498473136659106Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCT274910.2609411917469268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCGTTACCATTACTGAGTTTAGTAGATCGGAAGAGCACACGTCTGAA266710.2531578525729251Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC260370.24714000252863605Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATAC252700.23985973283783205TruSeq Adapter, Index 22 (97% over 37bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA246580.23405070408845519Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA245660.23317745140064033Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC236510.2244923839076995Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC218270.20717920018406646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG217010.20598322367684185Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG198440.18835680801084048No Hit
AACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACA194840.1849397322759129No Hit
TTTGGCAATGGTAGAACTCACACTAGATCGGAAGAGCACACGTCTGAACT194380.18450310593200553Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGA184790.17540039584924014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTC176170.16721839783949694Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCACAGGGTAGAACCACGGAAGATCGGAAGAGCACACGTCTGAACTCCA174640.1657661406521527Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC168440.15988117688644413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAGATCGGAAG167300.15879910290371707No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC162830.154556233866182No Hit
TGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAAC157900.14987673848473954Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGTAGATCGGAAGAGCACACGTCTGAACTCC157250.14925976647704428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACAC151990.14426703915323344No Hit
GAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACT151430.1437354940389114Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC148880.14131506539333769Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA148520.14097335781984494Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA148310.14077402840197414Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCAC147190.13971093817333002No Hit
CGCGACCTCAGATCAGACGTGAGATCGGAAGAGCACACGTCTGAACTCCA146120.1386953073298932Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT145980.13856242105131272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA145160.13778408713391255Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA144780.13742339580633686Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC144170.13684439130680748No Hit
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC144120.13679693192160014Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGCTGGGTTGAGAGGGCGAAGATCGGAAGAGCACACGTCTGAACTCC141210.13403479570253368Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT137520.13053229307423295No Hit
AAACCGTTACCATTACTGAGTTTAGATCGGAAGAGCACACGTCTGAACTC132050.12534023633255134Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA132040.12533074445550987Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC125660.1192749269030549No Hit
TGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCA124160.11785114534683509No Hit
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC124120.11781317783866921Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA123160.11690195764268854Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC121940.11574394864362976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCACTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA120250.11413982142362208Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTC115910.11002034678762608No Hit
TAGGAACTTCATACCGTGCTCTAGATCGGAAGAGCACACGTCTGAACTCC115250.10939388290288937Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA110060.1044675987183688Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTAGATCGGAAGAGCACACGTCTGAACTCC108520.10300584965398311Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)