FastQCFastQC Report
星期二 11 七月 2023
GDM3.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGDM3.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10465773
Total Bases523.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA279808226.735550255102993Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC8942438.544452473792429Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1837821.7560289144433Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA1744751.6671009394146044Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCG1600271.5290509358458282TruSeq Adapter, Index 22 (97% over 34bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1563391.4938122583014173Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1543011.4743392580748693Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1403321.3408660784062487Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1318671.2599833762876378Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1134781.0842772913190455Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCGCGTATGCCG1104491.0553353297458297TruSeq Adapter, Index 22 (97% over 34bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1067511.0200011026419167Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1040770.9944511504310287Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTT1036210.9900940905177286TruSeq Adapter, Index 22 (97% over 38bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCA986580.9426728441367876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC983300.9395388185851155Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC971560.9283213002995574Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC961510.9187185695695864Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA768980.7347570026600042Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG605290.5783519287108558Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA463620.4429868677640916Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA461730.44118098108950005TruSeq Adapter, Index 22 (97% over 38bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC460740.44023504045042827Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC457930.4375500978284165Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA426070.40710800817101617Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC420440.4017285679710424Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA381000.36404382170337535Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACTATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA371750.35520548744942204Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCA355710.33987933810526944Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCCACCGCTGCCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC339410.3243047599064111Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG334780.31988081530145934Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAGACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAAC330060.31537087609295555TruSeq Adapter, Index 22 (97% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG301110.2877092786170692No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC294500.28139345273397387Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC294250.2811545788352184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA283850.2712174246469898Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA264040.2522890569096043Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC244770.23387665679353067Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGAGATCGGAAGAGCACACGTCTGAACTCCA238090.22749394621878385Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC229460.21924801923374412Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTA219280.20952107407642034TruSeq Adapter, Index 22 (97% over 38bp)
CTATACGACCTGCTGCCTTTAGATCGGAAGAGCACACGTCTGAACTCCAG216500.20686479632225924Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA211120.20172423002104098Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC183570.17540032637818534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA172740.1650523090840973TruSeq Adapter, Index 2 (100% over 36bp)
ATTCAGACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAA167460.1600072923423812TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCGCGTA165960.15857404894984822TruSeq Adapter, Index 22 (97% over 38bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA162630.15539224861842504Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC162000.15479028639356118Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA149340.14269371216058288Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA146650.14012342900997377Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCC141670.13536506094676426Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA139870.1336451688757247Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCCGGCACCTCCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC139610.133396740021019Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGCACTGTGGGTACTTGCTAGATCGGAAGAGCACACGTCTGAACTCCAG139220.1330240967389604Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA136420.13034870907289886Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC135380.12935499365407602Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTT132220.12633562757380654TruSeq Adapter, Index 22 (97% over 38bp)
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC127970.12227477129496311No Hit
AAACCGTTACCACTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA125340.1197618178800553Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGAAGATCGGAAGAGCACACGTCTGAACTC123840.11832857448752232Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC122770.11730619420084881Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA119710.11438237768008155Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC117740.11250005135788822Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA117600.11236628197458516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA112020.10703461655436249Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAACTATGACTCTCTTAAGGAGATCGGAAGAGCACACGTCTGAACTCCAG110580.10565870289753083Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA108040.103231744086175Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTT105080.10040347712490993TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)