FastQCFastQC Report
星期二 11 七月 2023
GDM2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGDM2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10340357
Total Bases517 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA413915040.0290821680528Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC6896816.669798731320398Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA2385282.3067675516425594Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1766811.7086547398702Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1411341.3648851775620512Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1230861.1903457491844818Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1150391.1125244515252228Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1143301.1056678217202753Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1021700.9880703345155297Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC971790.9398031421932531Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC941620.9106261998497731Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA886130.8569626754666206Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC827760.8005139474391455Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG824610.7974676309531673No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC822800.7957172078294782No Hit
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG783010.7572369116462807Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCA770290.7449355955505211Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC645380.6241370583240018Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTTACTATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA588860.5694774368041644Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA582730.5635492082139911Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA568860.5501357448296997Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG480690.4648678957602721Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCA476150.4604773316820686Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC460420.4452650909441521Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC409060.39559562595372677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC359570.3477346091629138Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC343330.3320291552796485Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG341670.3304237948457679Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTG308260.2981134984024246TruSeq Adapter, Index 23 (97% over 37bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA300390.29050254261047276Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC299190.2893420410920049Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC261510.25290229341211334Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA248910.24071702746820056Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA243300.2352916828693632Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA240090.2321873413074616Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA238470.23062066425752997Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC229480.22192657371500807Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT220130.21288433271694582No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC216530.20940282816154218No Hit
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA209760.20285566542818592Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC204190.1974690042132975Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTATACGACCTGCTGCCTTTAGATCGGAAGAGCACACGTCTGAACTCCAG198430.19189859692465164Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA197610.19110558755369858Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCACTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA190140.18388146560123603Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA189560.18332055653397653Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA180280.1743460114578249Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGGGTGGCG178880.17299209301961238TruSeq Adapter, Index 23 (96% over 33bp)
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA161720.15639692130552166Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA158570.15335060481954346Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCC157930.1527316706763606Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA154830.14973370842031858Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGAAGATCGGAAGAGCACACGTCTGAACTC152800.1477705266849104Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCGTCACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA146520.14169723540492848Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTGCCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA145820.14102027618582222Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG145670.14087521349601373No Hit
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC145440.1406527840383074Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGTAGATCGGAAGAGCACACGTCTGAACTCC140260.13564328581692103Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCGTTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA135130.13068214182547083Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATCACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA131130.12681380343057788Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC129890.12561461852816105Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCAGATCGGAAGAGCACACGTCTGAACTC129310.12505370946090158Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGAGATCGGAAGAGCACACGTCTGAACT127390.12319690703135298Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCAAGTAATCCAGGATAGGAGATCGGAAGAGCACACGTCTGAACTCCAG117870.11399026165150779Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAACCGTTACCATTGCTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA112900.1091838511958533Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACTGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA110330.10669844377713458Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGAGATCGGAAGAGCACACGTCTGAACTCCA109300.10570234664044964Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCCTCCTGAATCTCGGATGGCG108970.10538320872287096TruSeq Adapter, Index 23 (96% over 33bp)
CAGTTGAACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCT104270.10083791110887176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCGTTACCATTACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC104210.10077988603294838Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCAAGTAATTCAGGATAGGTAGATCGGAAGAGCACACGTCTGAACTCCA103480.10007391427588042Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)