FastQCFastQC Report
星期二 11 七月 2023
GDM1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGDM1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10777725
Total Bases538.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA379175235.181376403647334Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGAGATCGGAAGAGCACACGTCTGAACTCC9771509.066384603429759Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGCAGATCGGAAGAGCACACGTCTGAACTCCA2301522.1354413848933795Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1937621.7978005562398374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1754471.627866734398957Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGAAGATCGGAAGAGCACACGTCTGAACTC1712811.5892129368674746Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC1705001.5819665096298154Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATCGGAAGAGCACACGTCTGAACTCCAG1388101.2879341419455406Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAAGATCGGAAGAGCACACGTCTGAACTCCA1232681.1437293120765282Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1211741.1243003509553269Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1155791.0723877256099965Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC1109031.0290019461435507Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTAGATCGGAAGAGCACACGTCTGAACTC1085441.0071142100953587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG932240.8649691841274479No Hit
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA906540.8411237065336145Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC879440.8159792535066537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAG670630.6222370676557436TruSeq Adapter, Index 16 (97% over 37bp)
TGAGGTAGTAGTTTGTACAGTAGATCGGAAGAGCACACGTCTGAACTCCA617470.5729131147807167Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAAAGATCGGAAGAGCACACGTCTGAACTCC562900.5222809080766116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCTTGGACTACATATGGTTGAGGGTTGTAAGATCGGAAGAGCACACGTC513470.47641779689127345No Hit
AAACCGTTACCATTACTGAGAAGATCGGAAGAGCACACGTCTGAACTCCA513120.47609305303299165Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGGCTTCCTCTCTGCAGGAAGATCGGAAGAGCACACGTCTGAACTCCA499090.4630754635138677Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACTATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA485120.4501135443704492Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGAGATCGGAAGAGCACACGTCTGAACTCCAG420550.39020294171543624Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC410940.3812864032066136Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC409230.3796997974990084Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCA375980.34884913096223924Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGAGATCGGAAGAGCACACGTCTGAACTCCA341940.31726547114534837Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTAGACTGAAGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA340520.31594793892031947Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGGGCAGAGAGCGAGACTAGATCGGAAGAGCACACGTCTGAACTCCA292970.27182916617375186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGAGTTAGATCGGAAGAGCACACGTCTGAACTCC283230.2627920085175675Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC263990.24494037470802046Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTAGATCGGAAGAGCACACGTCTGAACTC254780.23639497203723422Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCCCACTGCTAAATTTGACTGGCAGATCGGAAGAGCACACGTCTGAAC233650.2167897213929656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG232290.21552785954364212Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA229460.21290207348953513Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATTGCACGGTATCCATCTGTAAGATCGGAAGAGCACACGTCTGAACTCC228500.21201134747824796Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC228180.2117144388078189Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA220930.20498760174341063Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATTACTGGGTAGATCGGAAGAGCACACGTCTGAACTCCA212460.19712880037299152Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG197970.1836844046401258No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT189550.17587199524946126No Hit
GAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA188320.1747307525474996Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGCTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA188280.17469363896369597Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG187600.17406270803903423TruSeq Adapter, Index 16 (100% over 36bp)
ACATGGGTCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCC185900.17248538072737984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCACTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA171540.15916160414187597Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTATACGACCTGCTGCCTTTAGATCGGAAGAGCACACGTCTGAACTCCAG168290.15614612545783085Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGGGTGGCG167770.15566364886838363TruSeq Adapter, Index 16 (96% over 33bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC165660.15370590732274203Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACCGAGTAGATCGGAAGAGCACACGTCTGAACTCCA161590.1499296001707225Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGGT160320.14875124388495717TruSeq Adapter, Index 16 (97% over 37bp)
AAGCCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA155840.14459452249895038Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCAGCATTGTACAGGGCTATGAAGATCGGAAGAGCACACGTCTGAACTC152750.1417274981501198Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCC146540.13596561426460593Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCGTTACCATTACTGAGGAGATCGGAAGAGCACACGTCTGAACTCCA138560.12856145429578136Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCACTGTGGGTACTTGCTAGATCGGAAGAGCACACGTCTGAACTCCAG137640.1277078418682978Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTCTCACTACTGCACTTGACTAGTAGATCGGAAGAGCACACGTCTGAAC137330.12742021159381967Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCGTCACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA137070.12717897329909605Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTGCCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA133080.12347689331468376Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTGTGGTAGATCGGAAGAGCACACGTCTGAACTCCA122930.11405932142451213Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTAGATCGGAAGAGCACACGTCTGAACTCCA119980.11132219461899427Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCGTTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA119390.1107747692578907Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCGTTACCATCACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA118740.11017167352108168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC117980.10946651542881267No Hit
TCCTGTACTGAGCTGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCAG115350.10702629729372386Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTCGGTCCTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC113290.10511494772783682Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGCGACCTCAGATCAGACGTGTAGATCGGAAGAGCACACGTCTGAACTCC112660.1045304087829296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCGCGGT112210.10411288096513875TruSeq Adapter, Index 16 (97% over 37bp)
TCTCTCGGCTCCTCGCGGCTCGAGATCGGAAGAGCACACGTCTGAACTCC111660.1036025691878388Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)